Darrell J. Irvine

  • Professor of Materials Science and Engineering & Biological Engineering
  • Investigator, Howard Hughes Medical Institute
  • B.S. Engineering Physics, University of Pittsburgh, 1995
  • Ph.D. Polymer Science and Technology, MIT, 2000

Bio; Biotechnology; Nanotechnology; Polymers

Darrell J. Irvine

Research

The Irvine laboratory works at the interface of materials science and immunology. Synthetic materials can be applied in two major ways to 'immunobioengineering': (1) Synthetic materials can modulate the function of immune cells by mimicking signals derived from the immune system or foreign pathogens, both as a probe for cell function and as a tool for immunotherapy— both in vitro and in vivo; (2) Synthetic materials can be used to create in vitro and in vivo models of the microenvironment present in lymphoid and non-lymphoid tissues, to better understand immunobiology in health and disease. To meet these goals, engineering must be married to an in-depth appreciation for the biology of these problems. Their laboratory is thus deeply interdisciplinary in every project. Key to their approach is a balanced emphasis on both the engineering and immunological aspects of the problems the Irvine group has chosen to address: In each of these projects, state of the art chemistry, polymer science, physics and immunology are combined to address critical biological questions and medical challenges.

Recent News

Deshpande Center and J-WAFS announce fall 2017 research grants

October 17, 2017

The MIT Deshpande Center for Technological Innovation announced today the award of $768,000 in grants to 17 MIT research teams currently working on early-stage technologies. DMSE-related projects include: "Structured Nucleic Acid Nanoparticle Therapeutic Delivery Platform" — Mark Bathe,…  

Converging on cancer at the nanoscale

July 10, 2017

This summer, the Koch Institute for Integrative Cancer Research at MIT marks the first anniversary of the launch of the Marble Center for Cancer Nanomedicine. The Marble Center for Cancer Nanomedicine focuses on grand challenges in cancer detection, treatment, and monitoring that can…  

Fighting cancer with the power of immunity

October 26, 2016

Professor Darrell Irvine was a senior author alongside Dane Wittrup for a study published to the online edition of Nature Medicine on Oct. 24.  "Harnessing the body’s own immune system to destroy tumors is a tantalizing prospect that has yet to realize its full potential. However…  

Materializing the Future of Vaccines and Immunotherapy

January 25, 2016

The industries of immunotherapy and vaccination are on the brink of major breakthroughs, and many developments in the field are heavily focused on materials science. Safely modulating the immune system in patients with cancer is one of the biggest challenges at present, but developments in…  

More news about Darrell J. Irvine >>

Publications

2020

D. J. Irvine and Dane, E. L., “Enhancing cancer immunotherapy with nanomedicine”, Nature Reviews Immunology. Springer Science and Business Media LLC, 2020.
R. Veneziano et al., “Role of nanoscale antigen organization on B-cell activation probed using DNA origami”. Cold Spring Harbor Laboratory, 2020.
T. J. Moyer et al., “Engineered immunogen binding to alum adjuvant enhances humoral immunity”, Nature Medicine. Springer Science and Business Media LLC, 2020.
Y. Li et al., “Multifunctional oncolytic nanoparticles deliver self-replicating IL-12 RNA to eliminate established tumors and prime systemic immunity”, Nature Cancer. Springer Science and Business Media LLC, 2020.

2018

P. U. Atukorale et al., “Structure–Property Relationships of Amphiphilic Nanoparticles That Penetrate or Fuse Lipid Membranes”, Bioconjugate Chemistry, vol. 29. pp. 1131-1140, 2018.
Y. Zhang, Li, N., Suh, H., and Irvine, D. J., “Nanoparticle anchoring targets immune agonists to tumors enabling anti-cancer immunity without systemic toxicity”, Nature Communications, vol. 9. p. 6, 2018.

2017

W. L. Fotoran, Santangelo, R., de Mirand, B. N. M., Irvine, D. J., and Wunderlich, G., “DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins”, Molecular Therapy-Methods & Clinical Development, vol. 7. pp. 1-10, 2017.
Y. Fan, Kuai, R., Xup, Y., Ochyl, L. J., Irvine, D. J., and Moon, J. J., “Immunogenic Cell Death Amplified by Co-localized Adjuvant Delivery for Cancer Immunotherapy”, Nano Letters, vol. 17. pp. 7387-7393, 2017.
O. K. Appelbe, Moynihan, K. D., Flor, A., Rymut, N., Irvine, D. J., and Kron, S. J., “Radiation-enhanced delivery of systemically administered amphiphilic-CpG oligodeoxynucleotide”, Journal of Controlled Release, vol. 266. pp. 248-255, 2017.
D. Schmid et al., “T cell-targeting nanoparticles focus delivery of immunotherapy to improve antitumor immunity”, Nature Communications, vol. 8. p. 1747, 2017.
Y. He et al., “Synthetic Lift-off Polymer beneath Layer-by-Layer Films for Surface-Mediated Drug Delivery”, Acs Macro Letters, vol. 6. pp. 1320-1324, 2017.
E. Janssen et al., “DOCK8 enforces immunological tolerance by promoting IL-2 signaling and immune synapse formation in Tregs”, Jci Insight, vol. 2. p. e94298, 2017.
K. D. Moynihan and Irvine, D. J., “Roles for Innate Immunity in Combination Immunotherapies”, Cancer Research, vol. 77. pp. 5215-5221, 2017.
M. Pauthner et al., “Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches”, Immunity, vol. 46. p. 1073 - +, 2017.
L. Milling, Zhang, Y., and Irvine, D. J., “Delivering safer immunotherapies for cancer”, Advanced Drug Delivery Reviews, vol. 114. pp. 79-101, 2017.
W. Ngwa et al., “Smart Radiation Therapy Biomaterials”, International Journal of Radiation Oncology Biology Physics, vol. 97. pp. 624-637, 2017.
J. Li et al., “Structurally Programmed Assembly of Translation Initiation Nanoplex for Superior mRNA Delivery”, Acs Nano, vol. 11. pp. 2531-2544, 2017.
K. Rakhra et al., “Combination immunotherapy of an autochthonous murine lung cancer model expressing human CEA as a tumor-associated self-antigen”, Cancer Immunology Research, vol. 5. 2017.
Y. Zheng, Tang, L., Mabardi, L., Kumari, S., and Irvine, D. J., “Enhancing Adoptive Cell Therapy of Cancer through Targeted Delivery of Small-Molecule Immunomodulators to Internalizing or Noninternalizing Receptors”, Acs Nano, vol. 11. pp. 3089-3100, 2017.
K. D. Moynihan et al., “Eradication of large established tumors with combination immunotherapy engaging innate and adaptive immunity”, Cancer Immunology Research, vol. 5. 2017.
S. Kumari, Moynihan, K., and Irvine, D. J., “High-resolution visualization of immune response across the intact melanoma tumor”, Cancer Immunology Research, vol. 5. 2017.
Y. Zhang and Irvine, D. J., “Nanoparticle-mediated systemic delivery of immune agonists to tumors enabling anti-tumor efficacy without toxicity”, Cancer Immunology Research, vol. 5. 2017.
B. Jones et al., “Antigen recognition-triggered drug delivery mediated by nanocapsule-functionalized cytotoxic T-cells”, Biomaterials, vol. 117. pp. 44-53, 2017.
Y. -S. S. Yang et al., “High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry”, Nature Communications, vol. 8. p. 14069, 2017.
M. C. Hanson and Irvine, D. J., “Synthesis of Lymph Node-Targeting Adjuvants”, in Vaccine Adjuvants: Methods and Protocols, vol. 1494, Totowa: Humana Press Inc, 2017, pp. 145-152.

2016

K. L. Griffiths et al., “Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy”, Nature Communications, vol. 7. p. 13894, 2016.
A. Tzeng et al., “Temporally Programmed CD8 alpha(+) DC Activation Enhances Combination Cancer Immunotherapy”, Cell Reports, vol. 17. pp. 2503-2511, 2016.
K. D. Moynihan et al., “Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses”, Nature Medicine, vol. 22. p. 1402 - +, 2016.
H. H. Tam et al., “Sustained antigen availability during germinal center initiation enhances antibody responses to vaccination”, Proceedings of the National Academy of Sciences of the United States of America, vol. 113. pp. E6639 - E6648, 2016.
E. Janssen et al., “A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton”, Journal of Clinical Investigation, vol. 126. pp. 3837-3851, 2016.
J. M. Steichen et al., “HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies”, Immunity, vol. 45. pp. 483-496, 2016.
B. Jones et al., “A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4(+) T-Cells to Recognition by Cytotoxic T-Lymphocytes”, Plos Pathogens, vol. 12. p. e1005545, 2016.
T. J. Moyer, Zmolek, A. C., and Irvine, D. J., “Beyond antigens and adjuvants: formulating future vaccines”, Journal of Clinical Investigation, vol. 126. pp. 799-808, 2016.
D. J. Irvine, “A Receptor for All Occasions”, Cell, vol. 164. pp. 599-600, 2016.
L. Tang, Zheng, Y., Mabardi, L., and Irvine, D. J., “Carrier-free delivery of cytokine to specifically expand adoptively transferred T cells for enhanced cancer immunotherapy”, Cancer Immunology Research, vol. 4. 2016.
E. F. Zhu et al., “Characterization and combination immunotherapy treatment of an inducible autochthonous murine lung cancer model expressing human carcinoembryonic antigen (CEA) as a tumor-associated self-antigen”, Cancer Immunology Research, vol. 4. 2016.
D. J. Irvine, “Materializing the future of vaccines and immunotherapy”, Nature Reviews Materials, vol. 1. p. 15008, 2016.
R. J. Kimmerling et al., “A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages”, Nature Communications, vol. 7. p. 10220, 2016.
S. Kumari et al., “Specialized actin architecture mechanically tunes T cell synaptic contact lifetime.”, Molecular Biology of the Cell, vol. 27. 2016.

2015

E. L. Dane and Irvine, D. J., “Big thinking for adjuvants”, Nature Biotechnology, vol. 33. pp. 1146-1148, 2015.
K. B. Arnold, Szeto, G. L., Alter, G., Irvine, D. J., and Lauffenburger, D. A., “CD4(+) T cell-dependent and CD4(+) T cell-independent cytokine-chemokine network changes in the immune responses of HIV-infected individuals”, Science Signaling, vol. 8. p. ra104, 2015.
B. Huang, Abraham, W., Zheng, Y., Lopez, S. C. B., Luo, S. S., and Irvine, D. J., “Active targeting of chemotherapy to disseminated tumors using nanoparticle-carrying T cells”, Science Translational Medicine, vol. 7. p. 291ra94, 2015.
M. C. Hanson et al., “Nanoparticu late STING agonists are potent lymph node-targeted vaccine adjuvants”, Journal of Clinical Investigation, vol. 125. pp. 2532-2546, 2015.
G. L. Szeto et al., “Microfluidic squeezing for intracellular antigen loading in polyclonal B-cells as cellular vaccines”, Scientific Reports, vol. 5. p. 10276, 2015.
H. Liu and Irvine, D. J., “Guiding Principles in the Design of Molecular Bioconjugates for Vaccine Applications”, Bioconjugate Chemistry, vol. 26. pp. 791-801, 2015.
S. B. Kudchodkar, Tang, L., Jeng, E. K., Wong, H. C., Irvine, D. J., and Maus, M. V., “Improving CAR T Cell Efficacy for Solid Tumors By Nanogel-Based Delivery of Immunomodulatory Proteins”, Molecular Therapy, vol. 23. pp. S207 - S207, 2015.
E. F. Zhu et al., “Synergistic Innate and Adaptive Immune Response to Combination Immunotherapy with Anti-Tumor Antigen Antibodies and Extended Serum Half-Life IL-2”, Cancer Cell, vol. 27. pp. 489-501, 2015.
S. Wang et al., “Manipulating the Selection Forces during Affinity Maturation to Generate Cross-Reactive HIV Antibodies”, Cell, vol. 160. pp. 785-797, 2015.
M. C. Hanson et al., “Liposomal vaccines incorporating molecular adjuvants and intrastructural T-cell help promote the immunogenicity of HIV membrane-proximal external region peptides”, Vaccine, vol. 33. pp. 861-868, 2015.
M. Castellarnau et al., “Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis”, Small, vol. 11. pp. 489-498, 2015.
N. A. Hotaling, Tang, L., Irvine, D. J., and Babensee, J. E., “Biomaterial Strategies for Immunomodulation”, in Annual Review of Biomedical Engineering, Vol 17, vol. 17, 2015, pp. 317-349.
P. U. Atukorale et al., “Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes”, Nanoscale, vol. 7. pp. 11420-11432, 2015.

2014

Y. -S. Yang, Carney, R. P., Stellacci, F., and Irvine, D. J., “Enhancing Radiotherapy by Lipid Nanocapsule-Mediated Delivery of Amphiphilic Gold Nanoparticles to Intracellular Membranes”, Acs Nano, vol. 8. pp. 8992-9002, 2014.
S. Pejawar-Gaddy, Kovacs, J. M., Barouch, D. H., Chen, B., and Irvine, D. J., “Design of Lipid Nanocapsule Delivery Vehicles for Multivalent Display of Recombinant Env Trimers in HIV Vaccination”, Bioconjugate Chemistry, vol. 25. pp. 1470-1478, 2014.
R. B. Jones et al., “Histone Deacetylase Inhibitors Impair the Elimination of HIV-Infected Cells by Cytotoxic T-Lymphocytes”, Plos Pathogens, vol. 10. 2014.
M. C. Hanson, Bershteyn, A., Crespo, M. P., and Irvine, D. J., “Antigen Delivery by Lipid-Enveloped PLGA Microparticle Vaccines Mediated by in Situ Vesicle Shedding”, Biomacromolecules, vol. 15. pp. 2475-2481, 2014.
H. Liu et al., “Structure-based programming of lymph-node targeting in molecular vaccines”, Nature, vol. 507. p. 519 - +, 2014.
P. C. DeMuth, Min, Y., Irvine, D. J., and Hammond, P. T., “Implantable Silk Composite Microneedles for Programmable Vaccine Release Kinetics and Enhanced Immunogenicity in Transcutaneous Immunization”, Advanced Healthcare Materials, vol. 3. pp. 47-58, 2014.

2013

Y. Zheng, Stephan, M. T., Gai, A., Abraham, W., Shearer, A., and Irvine, D. J., “In vivo targeting of adoptively transferred T-cells with antibody- and cytokine-conjugated liposomes”, Journal of Controlled Release, vol. 172. pp. 426-435, 2013.
P. C. DeMuth et al., “Vaccine delivery with microneedle skin patches in nonhuman primates”, Nature Biotechnology, vol. 31. pp. 1082-1085, 2013.
J. Mata-Fink, Hanson, M. C., Falkowska, E., Burton, D., Irvine, D. J., and Wittrup, K., “Engineered Immunogens that Raise Antibodies to a Single Epitope”, Aids Research and Human Retroviruses, vol. 29. pp. A25 - A25, 2013.
M. Kim et al., “Immunogenicity of Membrane-bound HIV-1 gp41 Membrane-proximal External Region (MPER) Segments Is Dominated by Residue Accessibility and Modulated by Stereochemistry”, Journal of Biological Chemistry, vol. 288. pp. 31888-31901, 2013.
D. J. Irvine, Swartz, M. A., and Szeto, G. L., “Engineering synthetic vaccines using cues from natural immunity”, Nature Materials, vol. 12. pp. 978-990, 2013.
A. Drake, Joshi, N. S., Szeto, G. L., Zhu, E. F., Eisen, H. N., and Irvine, D. J., “Koch Institute Symposium on Cancer Immunology and Immunotherapy”, Cancer Immunology Research, vol. 1. pp. 217-222, 2013.
H. Liu and Irvine, D. J., “Structure-based programming of the immune response by molecular self-delivering vaccines”, Abstracts of Papers of the American Chemical Society, vol. 246. 2013.
R. C. Van Lehn et al., “Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers”, Nano Letters, vol. 13. pp. 4060-4067, 2013.
A. Li, Moon, J., and Irvine, D. J., “Stabilized lipid nanocapsules eliciting potent mucosal immune response”, Journal of Immunology, vol. 190. 2013.
M. E. Ackerman et al., “Enhanced Phagocytic Activity of HIV-Specific Antibodies Correlates with Natural Production of Immunoglobulins with Skewed Affinity for Fc gamma R2a and Fc gamma R2b”, Journal of Virology, vol. 87. pp. 5468-5476, 2013.
P. C. DeMuth, Moon, J. J., Suh, H., Hammond, P. T., and Irvine, D. J., “Layer-by-layer assembly of lipid nanocapsules on microneedles for enhanced transcutaneous vaccine delivery”, Abstracts of Papers of the American Chemical Society, vol. 245. 2013.
P. C. DeMuth et al., “Polymer multilayer tattooing for enhanced DNA vaccination”, Nature Materials, vol. 12. pp. 367-376, 2013.
X. Su, Yang, N., Wittrup, D., and Irvine, D. J., “Synergistic Antitumor Activity from Two-Stage Delivery of Targeted Toxins and Endosome-Disrupting Nanoparticles”, Biomacromolecules, vol. 14. pp. 1093-1102, 2013.
B. Kwong, Gai, A., Elkhader, J., Wittrup, D., and Irvine, D. J., “Localized Immunotherapy via Liposome-Anchored Anti-CD137+IL-2 Prevents Lethal Toxicity and Elicits Local and Systemic Antitumor Immunity”, Cancer Research, vol. 73. pp. 1547-1558, 2013.
J. Mata-Fink et al., “Rapid Conformational Epitope Mapping of Anti-gp120 Antibodies with a Designed Mutant Panel Displayed on Yeast”, Journal of Molecular Biology, vol. 425. pp. 444-456, 2013.
P. C. DeMuth, Garcia-Beltran, W. F., Ai-Ling, M. L., Hammond, P. T., and Irvine, D. J., “Composite Dissolving Microneedles for Coordinated Control of Antigen and Adjuvant Delivery Kinetics in Transcutaneous Vaccination”, Advanced Functional Materials, vol. 23. pp. 161-172, 2013.
Y. Wang and Irvine, D. J., “Convolution of chemoattractant secretion rate, source density, and receptor desensitization direct diverse migration patterns in leukocytes”, Integrative Biology, vol. 5. pp. 481-494, 2013.

2012

Y. J. Yamanaka et al., “Cellular Barcodes for Efficiently Profiling Single-Cell Secretory Responses by Microengraving”, Analytical Chemistry, vol. 84. pp. 10531-10536, 2012.
M. C. Hanson, Mata-Fink, J., Abraham, W., Wittrup, K. D., and Irvine, D. J., “Shaping humoral responses against mini-libraries of HIV env antigens via lipid nanoparticle vaccine delivery”, Retrovirology, vol. 9. 2012.
J. Mata-Fink, Hanson, M., Kriegsman, B., Irvine, D. J., and Wittrup, K., “Engineered gp120 immunogens that elicit VRC01-like antibodies by vaccination”, Retrovirology, vol. 9. 2012.
P. DeMuth, Huang, B., Min, Y., Barouch, D. H., Hammond, P., and Irvine, D. J., “Engineered microneedle arrays for transcutaneous HIV vaccine delivery”, Retrovirology, vol. 9. 2012.
P. C. DeMuth, Moon, J. J., Suh, H., Hammond, P. T., and Irvine, D. J., “Releasable Layer-by-Layer Assembly of Stabilized Lipid Nanocapsules on Microneedles for Enhanced Transcutaneous Vaccine Delivery”, Acs Nano, vol. 6. pp. 8041-8051, 2012.
D. J. Irvine and Liu, H., “Dynamically stabilized oligonucleotide and peptide micelles for lymphoid tissue targeting: Designing vaccines one molecule at a time”, Abstracts of Papers of the American Chemical Society, vol. 244. 2012.
M. T. Stephan, Stephan, S. B., Bak, P., Chen, J., and Irvine, D. J., “Synapse-directed delivery of immunomodulators using T-cell-conjugated nanoparticles”, Biomaterials, vol. 33. pp. 5776-5787, 2012.
J. J. Moon, Huang, B., and Irvine, D. J., “Engineering Nano- and Microparticles to Tune Immunity”, Advanced Materials, vol. 24. pp. 3724-3746, 2012.
C. Jewell, Lopez, S. B., and Irvine, D. J., “Engineering the Lymph Node Microenvironment via Intranodal Controlled Release Vaccine Depots”, Journal of Immunology, vol. 188. 2012.
H. Liu and Irvine, D. J., “Immunostimulatory properties of dynamic oligonucleotide micelles”, Journal of Immunology, vol. 188. 2012.
B. Kwong, Liu, H., and Irvine, D. J., “Intratumoral delivery of liposome-anchored anti-CD137 and IL-2 induces highly potent local and systemic anti-tumor immunity while minimizing toxic inflammatory side effects”, Journal of Immunology, vol. 188. 2012.
J. Moon, Suh, H., Li, A., Yadava, A., and Irvine, D. J., “Nanoparticle vaccines enhance humoral responses to a malaria antigen by expanding Tfh cells and inducing germinal center formation”, Journal of Immunology, vol. 188. 2012.
M. Stephan, Bak, S., Stephan, S., Chen, J., and Irvine, D. J., “Synapse-directed delivery of immune-stimulants using T-cell-conjugated nanoparticles”, Journal of Immunology, vol. 188. 2012.
A. Li et al., “Eliciting mucosal and systemic CD8+T-cell responses via pulmonary vaccination with synthetic nanoparticles”, Journal of Immunology, vol. 188. 2012.
B. Huang and Irvine, D. J., “Targeted chemotherapeutic delivery to disseminated lymphoma tumors via adoptive transfer of drug nanoparticle-carrying T cells”, Journal of Immunology, vol. 188. 2012.
P. DeMuth, Huang, B., Min, Y., Hammond, P., and Irvine, D. J., “Engineered microneedle arrays for transcutaneous HIV vaccine delivery”, Journal of Immunology, vol. 188. 2012.
D. J. Irvine, “Engineering nanoparticle interactions with cells via synergies in biochemistry and physical chemistry[p]”, Abstracts of Papers of the American Chemical Society, vol. 243. 2012.
A. Bershteyn et al., “Robust IgG responses to nanograms of antigen using a biomimetic lipid-coated particle vaccine”, Journal of Controlled Release, vol. 157. pp. 354-365, 2012.
J. J. Moon, Suh, H., Polhemus, M. E., Ockenhouse, C. F., Yadava, A., and Irvine, D. J., “Antigen-Displaying Lipid-Enveloped PLGA Nanoparticles as Delivery Agents for a Plasmodium vivax Malaria Vaccine”, Plos One, vol. 7. 2012.
J. J. Moon, Suh, H., Li, A. V., Ockenhouse, C. F., Yadava, A., and Irvine, D. J., “Enhancing humoral responses to a malaria antigen with nanoparticle vaccines that expand T-fh cells and promote germinal center induction”, Proceedings of the National Academy of Sciences of the United States of America, vol. 109. pp. 1080-1085, 2012.
C. M. Metallo et al., “Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia”, Nature, vol. 481. pp. 380 - U166, 2012.

2011

C. M. Jewell, Lopez, S. C. B., and Irvine, D. J., “In situ engineering of the lymph node microenvironment via intranodal injection of adjuvant-releasing polymer particles”, Proceedings of the National Academy of Sciences of the United States of America, vol. 108. pp. 15745-15750, 2011.
N. Martinez-Martin et al., “T Cell Receptor Internalization from the Immunological Synapse Is Mediated by TC21 and RhoG GTPase-Dependent Phagocytosis”, Immunity, vol. 35. pp. 208-222, 2011.
B. Kwong, Liu, H., and Irvine, D. J., “Induction of potent anti-tumor responses while eliminating systemic side effects via liposome-anchored combinatorial immunotherapy”, Biomaterials, vol. 32. pp. 5134-5147, 2011.
V. Dahirel et al., “Coordinate linkage of HIV evolution reveals regions of immunological vulnerability”, Proceedings of the National Academy of Sciences of the United States of America, vol. 108. pp. 11530-11535, 2011.
M. -L. De Temmerman, Rejman, J., Demeester, J., Irvine, D. J., Gander, B., and De Smedt, S. C., “Particulate vaccines: on the quest for optimal delivery and immune response”, Drug Discovery Today, vol. 16. pp. 569-582, 2011.
M. J. Palmer, Mahajan, V. S., Chen, J., Irvine, D. J., and Lauffenburger, D. A., “Signaling thresholds govern heterogeneity in IL-7-receptor-mediated responses of naive CD8(+) T cells”, Immunology and Cell Biology, vol. 89. pp. 581-594, 2011.
J. -W. Yoo, Irvine, D. J., Discher, D. E., and Mitragotri, S., “Bio-inspired, bioengineered and biomimetic drug delivery carriers”, Nature Reviews Drug Discovery, vol. 10. pp. 521-535, 2011.
Y. Wang and Irvine, D. J., “Engineering chemoattractant gradients using chemokine-releasing polysaccharide microspheres”, Biomaterials, vol. 32. pp. 4903-4913, 2011.
X. Su, Fricke, J., Kavanagh, D. G., and Irvine, D. J., “In Vitro and in Vivo mRNA Delivery Using Lipid-Enveloped pH-Responsive Polymer Nanoparticles”, Molecular Pharmaceutics, vol. 8. pp. 774-787, 2011.
M. T. Stephan and Irvine, D. J., “Enhancing cell therapies from the outside in: Cell surface engineering using synthetic nanomaterials”, Nano Today, vol. 6. pp. 309-325, 2011.
D. J. Irvine, “DRUG DELIVERY One nanoparticle, one kill”, Nature Materials, vol. 10. pp. 342-343, 2011.
A. T. Melvin, Welf, E. S., Wang, Y., Irvine, D. J., and Haugh, J. M., “In Chemotaxing Fibroblasts, Both High-Fidelity and Weakly Biased Cell Movements Track the Localization of PI3K Signaling”, Biophysical Journal, vol. 100. pp. 1893-1901, 2011.
M. E. Ackerman et al., “A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples”, Journal of Immunological Methods, vol. 366. pp. 8-19, 2011.
J. J. Moon et al., “Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses”, Nature Materials, vol. 10. pp. 243-251, 2011.
H. Liu, Kwong, B., and Irvine, D. J., “Membrane Anchored Immunostimulatory Oligonucleotides for In Vivo Cell Modification and Localized Immunotherapy”, Angewandte Chemie-International Edition, vol. 50. pp. 7052-7055, 2011.
C. M. Jewell, Jung, J. -M., Atukorale, P. U., Carney, R. P., Stellacci, F., and Irvine, D. J., “Oligonucleotide Delivery by Cell-Penetrating "Striped" Nanoparticles”, Angewandte Chemie-International Edition, vol. 50. pp. 12312-12315, 2011.

2010

P. C. DeMuth, Su, X., Samuel, R. E., Hammond, P. T., and Irvine, D. J., “Nano-Layered Microneedles for Transcutaneous Delivery of Polymer Nanoparticles and Plasmid DNA”, Advanced Materials, vol. 22. p. 4851 - +, 2010.
M. T. Stephan, Moon, J. J., Um, S. H., Bershteyn, A., and Irvine, D. J., “Therapeutic Cell Engineering Using Surface-Conjugated Synthetic Nanoparticles”, Journal of Immunotherapy, vol. 33. pp. 866-866, 2010.
M. T. Stephan, Moon, J. J., Um, S. H., Bershteyn, A., and Irvine, D. J., “Therapeutic cell engineering with surface-conjugated synthetic nanoparticles”, Nature Medicine, vol. 16. pp. 1035 - U135, 2010.
A. Swiston, Gilbert, J., Irvine, D. J., Cohen, R., and Rubner, M. F., “Injectable cellular "Backpacks" lead to cell aggregate self-assembly”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
B. Huang and Irvine, D. J., “Surface functionalization of lymphocytes as chemotherapeutic controlled release carriers”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
B. Kwong and Irvine, D. J., “Synthesis and characterization of antibody-nanoparticle conjugates for locally sequestered tumor immunotherapy”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
J. J. Moon et al., “Synthesis and characterization of interbilayer-crosslinked multilamellar vesicles for vaccine delivery”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
A. Bershteyn and Irvine, D. J., “Synthesis and characterization of lipid-coated micro- and nano-particles co-displaying antigen and adjuvant as dose-sparing vaccine delivery vehicles”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
P. C. DeMuth, Hammond, P. T., and Irvine, D. J., “Transcutaneous delivery of plasmid DNA and degradable polymer nanoparticles from multilayer-coated microneedle arrays”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
D. J. Irvine, “Combining cell therapy and nanoparticle drug delivery: Chemical cell surface engineering on live cells using nanoparticles”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
X. Su, Kavanagh, D., and Irvine, D. J., “Cytosolic delivery mediated via electrostatic surface binding of mRNA to degradable lipid-coated polymeric nanoparticles”, Abstracts of Papers of the American Chemical Society, vol. 240. 2010.
A. J. Swiston, Gilbert, J. B., Irvine, D. J., Cohen, R. E., and Rubner, M. F., “Freely Suspended Cellular "Backpacks" Lead to Cell Aggregate Self-Assembly”, Biomacromolecules, vol. 11. pp. 1826-1832, 2010.
H. Phee et al., “Regulation of thymocyte positive selection and motility by GIT2”, Nature Immunology, vol. 11. pp. 503 - U70, 2010.
R. P. Carney, Jung, J. -M., Irvine, D. J., and Stellacci, F., “All hydrophilic surface moieties for cell-penetrating nanoparticles”, Abstracts of Papers of the American Chemical Society, vol. 239. 2010.

2009

Y. Hor, Stern, P. J., Hynes, R. O., and Irvine, D. J., “Engulfing tumors with synthetic extracellular matrices for cancer immunotherapy”, Biomaterials, vol. 30. pp. 6757-6767, 2009.
X. Su, Kim, B. -S., Kim, S. R., Hammond, P. T., and Irvine, D. J., “Layer-by-Layer-Assembled Multilayer Films for Transcutaneous Drug and Vaccine Delivery”, Acs Nano, vol. 3. pp. 3719-3729, 2009.
Y. Hori, Winans, A. M., and Irvine, D. J., “Modular injectable matrices based on alginate solution/microsphere mixtures that gel in situ and co-deliver immunomodulatory factors”, Acta Biomaterialia, vol. 5. pp. 969-982, 2009.
R. A. Maldonado et al., “Control of T helper cell differentiation through cytokine receptor inclusion in the immunological synapse”, Journal of Experimental Medicine, vol. 206. pp. 877-892, 2009.
A. C. Miller, Bershteyn, A., Tan, W., Hammond, P. T., Cohen, R. E., and Irvine, D. J., “Block Copolymer Micelles as Nanocontainers for Controlled Release of Proteins from Biocompatible Oil Phases”, Biomacromolecules, vol. 10. pp. 732-741, 2009.
Y. Hu et al., “Cytosolic Delivery Mediated via Electrostatic Surface Binding of Protein, Virus, or siRNA Cargos to pH-Responsive Core-Shell Gel Particles”, Biomacromolecules, vol. 10. pp. 756-765, 2009.
A. Verma, Uzun, O., Irvine, D. J., and Stellacci, F., “Cell membrane penetration by nanoparticles: The role of ligand shell structure”, Abstracts of Papers of the American Chemical Society, vol. 237. 2009.
D. J. Irvine, “Cells as engineering substrates: Surface modification and interior loading of living microcapsules”, Abstracts of Papers of the American Chemical Society, vol. 237. 2009.
A. Bershteyn, Chaparro, J. P., Riley, E. B., Yao, R. S., Zachariah, R. S., and Irvine, D. J., Lipid-Coated Biodegradable Particles as "Synthetic Pathogens" for Vaccine Engineering. 2009.

2008

A. J. Swiston, Cheng, C., Um, S. H., Irvine, D. J., Cohen, R. E., and Rubner, M. F., “Surface Functionalization of Living Cells with Multilayer Patches”, Nano Letters, vol. 8. pp. 4446-4453, 2008.
Y. Hori, Winans, A. M., Huang, C. C., Horrigan, E. M., and Irvine, D. J., “Injectable dendritic cell-carrying alginate gels for immunization and immunotherapy”, Biomaterials, vol. 29. pp. 3671-3682, 2008.
D. J. Irvine, “PMSE 55-Applying nanomaterials to immunology”, Abstracts of Papers of the American Chemical Society, vol. 236. 2008.
A. Verma et al., “Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles”, Nature Materials, vol. 7. pp. 588-595, 2008.
A. N. Stachowiak and Irvine, D. J., “Inverse opal hydrogel-collagen composite scaffolds as a supportive microenvironment for immune cell migration”, Journal of Biomedical Materials Research Part A, vol. 85A. pp. 815-828, 2008.
M. J. Palmer, Mahajan, V. S., Trajman, L. C., Irvine, D. J., Lauffenburger, D. A., and Chen, J., “Interleukin-7 receptor signaling network: An integrated systems perspective”, Cellular & Molecular Immunology, vol. 5. pp. 79-89, 2008.
D. J. Irvine, Stachowiak, A. N., and Hori, Y., “Lymphoid tissue engineering: Invoking lymphoid tissue neogenesis in immunotherapy and models of immunity”, Seminars in Immunology, vol. 20. pp. 137-146, 2008.
A. C. Miller, Bennett, R. D., Hammond, P. T., Irvine, D. J., and Cohen, R. E., “Functional nanocavity arrays via amphiphilic block copolymer thin films”, Macromolecules, vol. 41. pp. 1739-1744, 2008.
A. Bershteyn et al., “Polymer-supported lipid shells, onions, and flowers”, Soft Matter, vol. 4. pp. 1787-1791, 2008.

2007

Y. Hu et al., “Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-Responsive core-shell nanoparticles”, Nano Letters, vol. 7. pp. 3056-3064, 2007.
D. J. Irvine, Doh, J., and Huang, B., “Patterned surfaces as tools to study ligand recognition and synapse formation by T cells”, Current Opinion in Immunology, vol. 19. pp. 463-469, 2007.
D. J. Irvine and Doh, J., “Synthetic surfaces as artificial antigen presenting cells in the study of T cell receptor triggering and immunological synapse formation”, Seminars in Immunology, vol. 19. pp. 245-254, 2007.
D. J. Irvine, “Engineering immunity: Using polymers to probe and prod cells of the immune system”, Abstracts of Papers of the American Chemical Society, vol. 233. 2007.

2006

R. D. Bennett, Hart, A. J., Miller, A. C., Hammond, P. T., Irvine, D. J., and Cohen, R. E., “Creating patterned carbon nanotube catalysts through the microcontact printing of block copolymer micellar thin films”, Langmuir, vol. 22. pp. 8273-8276, 2006.
A. N. Stachowiak, Wang, Y., Huang, Y. -C., and Irvine, D. J., “Homeostatic lymphoid chemokines synergize with adhesion ligands to trigger T and B lymphocyte chemokinesis”, Journal of Immunology, vol. 177. pp. 2340-2348, 2006.
H. Kim, Cohen, R. E., Hammond, P. T., and Irvine, D. J., “Live lymphocyte arrays for biosensing”, Advanced Functional Materials, vol. 16. pp. 1313-1323, 2006.
J. Doh and Irvine, D. J., “Immunological synapse arrays: Patterned protein surfaces that modulate immunological synapse structure formation in T cells”, Proceedings of the National Academy of Sciences of the United States of America, vol. 103. pp. 5700-5705, 2006.
S. Jain, Navot, B., Yap, W. T., and Irvine, D. J., “Compound delivery microparticles for chemoattraction of dendritic cells followed by co-delivery of antigen and activation signals to DCs”, Journal of Immunology, vol. 176. pp. S255 - S255, 2006.
A. N. Stachowiak, Wang, Y., and Irvine, D. J., “Homeostatic lymphoid chemokines syngergize with adhesion ligands to trigger T and B lymphocyte chemokinesis”, Journal of Immunology, vol. 176. pp. S30 - S30, 2006.
D. J. Irvine and Doh, J., “Immunological synapse arrays: Patterned protein surfaces that modulate immunological synapse structure formation in T cells”, Journal of Immunology, vol. 176. pp. S172 - S172, 2006.
R. A. Maldonado, Irvine, D. J., and Glimcher, L. H., “A role for the immunological synapse in lineage commitment of CD4 lymphocytes”, Journal of Immunology, vol. 176. pp. S13 - S13, 2006.
J. S. Katz, Doh, J., and Irvine, D. J., “Composition-tunable properties of amphiphilic comb copolymers containing protected methacrylic acid groups for multicomponent protein patterning”, Langmuir, vol. 22. pp. 353-359, 2006.

2005

R. D. Bennett, Miller, A. C., Kohen, N. T., Hammond, P. T., Irvine, D. J., and Cohen, R. E., “Strategies for controlling the planar arrangement of block copolymer micelles and inorganic nanoclusters”, Macromolecules, vol. 38. pp. 10728-10735, 2005.
S. Jain, Yap, W. T., and Irvine, D. J., “Synthesis of protein-loaded hydrogel particles in an aqueous two-phase system for coincident antigen and CpG oligonucleotide delivery to antigen-presenting cells”, Biomacromolecules, vol. 6. pp. 2590-2600, 2005.
J. Doh and Irvine, D. J., “Aqueous-processible photoresist polymer for multiple protein patterning: synthesis, characterization, and application to T cell activation”, Abstracts of Papers of the American Chemical Society, vol. 230. pp. U3602 - U3602, 2005.
D. J. Irvine, Jain, S., Yap, W. T., and Huang, Y. C., “Colloidal micelles as multifunctional vaccines”, Abstracts of Papers of the American Chemical Society, vol. 230. pp. U4156 - U4157, 2005.
X. J. Zhao, Jain, S., Larman, H. B., Gonzalez, S., and Irvine, D. J., “Directed cell migration via chemoattractants released from degradable microspheres”, Biomaterials, vol. 26. pp. 5048-5063, 2005.
J. R. Greenland et al., “beta-amino ester polymers facilitate in vivo DNA transfection and adjuvant plasmid DNA immunization”, Molecular Therapy, vol. 12. pp. 164-170, 2005.
D. J. Irvine, Stachowiak, A., and Bershteyn, A., “Factors controlling naive T cell motility in an in vitro model of the lymphoid microenviromment”, Faseb Journal, vol. 19. pp. A927 - A927, 2005.
R. A. Maldonado, Irvine, D. J., Schreiber, R., and Glimcher, L. H., “A role for the immunological synapse in lineage commitment of CD4 lymphocytes”, Faseb Journal, vol. 19. pp. A900 - A900, 2005.
A. N. Stachowiak, Bershteyn, A., Tzatzalos, E., and Irvine, D. J., “Bioactive hydrogels with an ordered cellular structure combine interconnected macroporosity and robust mechanical properties”, Advanced Materials, vol. 17. p. 399 - +, 2005.

2004

R. A. Maldonado, Irvine, D. J., Schreiber, R., and Glimcher, L. H., “A role for the immunological synapse in lineage commitment of CD4 lymphocytes”, Nature, vol. 431. pp. 527-532, 2004.
D. J. Irvine and Doh, J. S., “Mimicking the immunological synapse with multicomponent protein-patterned surfaces.”, Abstracts of Papers of the American Chemical Society, vol. 228. pp. U392 - U392, 2004.
J. Doh and Irvine, D. J., “Photogenerated polyelectrolyte bilayers from an aqueous-processible photoresist for multicomponent protein patterning”, Journal of the American Chemical Society, vol. 126. pp. 9170-9171, 2004.
H. Kim, Doh, J., Irvine, D. J., Cohen, R. E., and Hammond, P. T., “Large area two-dimensional B cell arrays for sensing and cell-sorting applications”, Biomacromolecules, vol. 5. pp. 822-827, 2004.
S. Jain and Irvine, D. J., “Rational design of a vaccine for chemoattraction and "programming" of dendritic cells”, Faseb Journal, vol. 18. pp. A820 - A820, 2004.
A. Stachowiak and Irvine, D. J., “Toward an in vitro model of the lymph node T zone”, Faseb Journal, vol. 18. pp. A429 - A429, 2004.

2003

M. M. Davis et al., “Dynamics of cell surface molecules during T cell recognition”, Annual Review of Biochemistry, vol. 72. pp. 717-742, 2003.
D. J. Irvine, Stachowiak, A., and Jain, S., “Engineering biomaterials for control of immune cell functions”, in Thermec\textquoteright2003, Pts 1-5, vol. 426-4, 2003, pp. 3213-3218.

2002

J. Norman, Moratti, S. C., Slark, A. T., Irvine, D. J., and Jackson, A. T., “Synthesis of well-defined macromonomers by sequential ATRP-catalytic chain transfer and copolymerization with ethyl acrylate”, Macromolecules, vol. 35. pp. 8954-8961, 2002.
W. C. Moss, Irvine, D. J., Davis, M. M., and Krummel, M. F., “Quantifying signaling-induced reorientation of T cell receptors during immunological synapse formation”, Proceedings of the National Academy of Sciences of the United States of America, vol. 99. pp. 15024-15029, 2002.
L. Y. Koo, Irvine, D. J., Mayes, A. M., Lauffenburger, D. A., and Cima, L., “Co-regulation of cell adhesion by nanoscale RGD organization and mechanical stimulus”, Journal of Cell Science, vol. 115. pp. 1423-1433, 2002.
D. J. Irvine, Hue, K. A., Mayes, A. M., and Cima, L., “Simulations of cell-surface integrin binding to nanoscale-clustered adhesion ligands”, Biophysical Journal, vol. 82. pp. 120-132, 2002.

2001

A. M. Mayes et al., “Tailoring surface interactions via self-organizing, amphiphilic comb copolymers.”, Abstracts of Papers of the American Chemical Society, vol. 221. pp. U391 - U391, 2001.
D. J. Irvine, Ruzette, A. V. G., Mayes, A. M., and Cima, L., “Nanoscale clustering of RGD peptides at surfaces using comb polymers. 2. Surface segregation of comb polymers in polylactide”, Biomacromolecules, vol. 2. pp. 545-556, 2001.
D. J. Irvine, Mayes, A. M., and Cima, L., “Nanoscale clustering of RGD peptides at surfaces using comb polymers. 1. Synthesis and characterization of comb thin films”, Biomacromolecules, vol. 2. pp. 85-94, 2001.

2000

P. Banerjee, Irvine, D. J., Mayes, A. M., and Cima, L., “Polymer latexes for cell-resistant and cell-interactive surfaces”, Journal of Biomedical Materials Research, vol. 50. pp. 331-339, 2000.

1999

T. D. Johnson, Irvine, D. J., Mayes, A., Wells, A., and Cima, L., “Stimulation of neural differentiation with tethered epidermal growth factor”, Molecular Biology of the Cell, vol. 10. p. 39A - 39A, 1999.

1998

D. J. Irvine, Mayes, A. M., and Cima, L., “Design of polymeric cell-signaling surfaces and their application as biomaterials.”, Abstracts of Papers of the American Chemical Society, vol. 216. pp. U795 - U795, 1998.
D. J. Irvine, Mayes, A. M., Satija, S. K., Barker, J. G., Sofia-Allgor, S. J., and Cima, L., “Comparison of tethered star and linear poly(ethylene oxide) for control of biomaterials surface properties”, Journal of Biomedical Materials Research, vol. 40. pp. 498-509, 1998.
A. M. Mayes, Irvine, D. J., and Cima, L., “Tailoring polymer surfaces for controlled cell behavior”, in Biomaterials Regulating Cell Function and Tissue Development, vol. 530, 1998, pp. 73-84.

1996

D. J. Irvine, Mayes, A. M., and Cima, L., “Self-consistent field analysis of grafted star polymers”, Macromolecules, vol. 29. pp. 6037-6043, 1996.