Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression

TitleTumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Publication TypeJournal Article
Year of Publication2016
AuthorsOudin, MJ, Jonas, O, Kosciuk, T, Broye, LC, Guido, BC, Wyckoff, J, Riquelme, D, Lamar, JM, Asokan, SB, Whittaker, C, Ma, D, Langer, R, Cima, MJ, Wisinski, KB, Hynes, RO, Lauffenburger, DA, Keely, PJ, Bear, JE, Gertler, FB
JournalCancer Discovery
Volume6
Issue5
Pagination516 - 531
Date Published2016/05//
ISBN Number2159-8274
Keywordsbreast-cancer, carcinoma-cells, chemotaxis, collagen, expression, fibronectin, invasion, mammary-tumors, protein mena, transendothelial migration
Abstract

Fibronectin (FN) is a major component of the tumor microenvironment, but its role in promoting metastasis is incompletely understood. Here, we show that FN gradients elicit directional movement of breast cancer cells, in vitro and in vivo. Haptotaxis on FN gradients requires direct interaction between alpha 5 beta 1 integrin and MENA, an actin regulator, and involves increases in focal complex signaling and tumor cell-mediated extracellular matrix (ECM) remodeling. Compared with MENA, higher levels of the prometastatic MENA(INV) isoform associate with alpha 5, which enables 3-D haptotaxis of tumor cells toward the high FN concentrations typically present in perivascular space and in the periphery of breast tumor tissue. MENA(INV) and FN levels were correlated in two breast cancer cohorts, and high levels of MENA(INV) were significantly associated with increased tumor recurrence as well as decreased patient survival. Our results identify a novel tumor cell-intrinsic mechanism that promotes metastasis through ECM remodeling and ECM-guided directional migration. SIGNIFICANCE: Here, we provide new insight into how tumor cell: ECM interactions generate signals and structures that promote directed tumor cell migration, a critical component of metastasis. Our results identify a tumor cell-intrinsic mechanism driven by the actin regulatory protein MENA that promotes ECM remodeling and haptotaxis along FN gradients. Cancer Discov; 6(5); 516-31. (C) 2016 AACR.

Short TitleCancer Discov.