Multiphase Oscillatory Flow Strategy for in Situ Measurement and Screening of Partition Coefficients

TitleMultiphase Oscillatory Flow Strategy for in Situ Measurement and Screening of Partition Coefficients
Publication TypeJournal Article
Year of Publication2015
AuthorsAbolhasani, M, Coley, CW, Jensen, KF
JournalAnalytical Chemistry
Volume87
Issue21
Pagination11130 - 11136
Date Published2015/11/03/
Abstract

Taking advantage of the difference between the surface energies of aqueous and organic solvents on a Teflon substrate, a fully automated small-scale strategy is developed on the basis of gas-driven oscillatory motion of a biphasic slug for high-throughput in situ measurement and screening of partition coefficients of organic substances between aqueous and organic phases. The developed oscillatory flow strategy enables single partition coefficient data point measurement within 8 min (including the sample preparation time) which is 360 times faster than the conventional "shake-flask" method, while using less than a 30 mu L volume of the two phases and 9 nmol of the target organic substance. The developed multiphase strategy is validated using a conventional shake-flask technique. Finally, the developed strategy is extended to include automated screening of partition coefficients at physiological temperature.