|Title||High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Yang, Y-SSabrina, Atukorale, PU, Moynihan, KD, Bekdemir, A, Rakhra, K, Tang, L, Stellacci, F, Irvine, DJ|
|Keywords||ablation, delivery, gold nanoparticles, icp-ms, in-vivo, quantification, spectrometry, technology, therapy, tomography|
Inorganic nanoparticles (NPs) are studied as drug carriers, radiosensitizers and imaging agents, and characterizing nanoparticle biodistribution is essential for evaluating their efficacy and safety. Tracking NPs at the single-cell level with current technologies is complicated by the lack of reliable methods to stably label particles over extended durations in vivo. Here we demonstrate that mass cytometry by time-of-flight provides a label-free approach for inorganic nanoparticle quantitation in cells. Furthermore, mass cytometry can enumerate AuNPs with a lower detection limit of B10 AuNPs (3 nm core size) in a single cell with tandem multiparameter cellular phenotyping. Using the cellular distribution insights, we selected an amphiphilic surface ligand-coated AuNP that targeted myeloid dendritic cells in lymph nodes as a peptide antigen carrier, substantially increasing the efficacy of a model vaccine in a B16-OVA melanoma mouse model. This technology provides a powerful new level of insight into nanoparticle fate in vivo.
|Short Title||Nat. Commun.|