Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

TitleEx Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
Publication TypeJournal Article
Year of Publication2015
AuthorsSharei, A, Trifonova, R, Jhunjhunwala, S, Hartoularos, GC, Eyerman, AT, Lytton-Jean, A, Angin, M, Sharma, S, Poceviciute, R, Mao, S, Heimann, M, Liu, S, Talkar, T, Khan, OF, Addo, M, von Andrian, UH, Anderson, DG, Langer, R, Lieberman, J, Jensen, KF
JournalPlos One
Volume10
Issue4
Paginatione0118803
Date Published2015/04/13/
Abstract

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.