A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton

TitleA DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton
Publication TypeJournal Article
Year of Publication2016
AuthorsJanssen, E, Tohme, M, Hedayat, M, Leick, M, Kumari, S, Ramesh, N, Massaad, MJ, Ullas, S, Azcutia, V, Goodnow, CC, Randall, KL, Qiao, Q, Wu, H, Al-Herz, W, Cox, D, Hartwig, J, Irvine, DJ, Luscinskas, FW, Geha, RS
JournalJournal of Clinical Investigation
Volume126
Issue10
Pagination3837 - 3851
Date Published2016/10//
ISBN Number0021-9738
Keywordsactivation, arp2/3 complex, cdc42, dock8 mutations, immunological synapse, phosphatidylinositol 4,5-bisphosphate, polymerization, syndrome protein wasp, wip, wiskott-aldrich-syndrome
Abstract

Wiskott-Aldrich syndrome (WAS) is associated with mutations in the WAS protein (WASp), which plays a critical role in the initiation of T cell receptor-driven (TCR-driven) actin polymerization. The clinical phenotype of WAS includes susceptibility to infection, allergy, autoimmunity, and malignancy and overlaps with the symptoms of dedicator of cytokinesis 8 (DOCK8) deficiency, suggesting that the 2 syndromes share common pathogenic mechanisms. Here, we demonstrated that the WASp-interacting protein (WIP) bridges DOCK8 to WASp and actin in T cells. We determined that the guanine nucleotide exchange factor activity of DOCK8 is essential for the integrity of the subcortical actin cytoskeleton as well as for TCR-driven WASp activation, F-actin assembly, immune synapse formation, actin foci formation, mechanotransduction, T cell transendothelial migration, and homing to lymph nodes, all of which also depend on WASp. These results indicate that DOCK8 and WASp are in the same signaling pathway that links TCRs to the actin cytoskeleton in TCR-driven actin assembly. Further, they provide an explanation for similarities in the clinical phenotypes of WAS and DOCK8 deficiency.

Short TitleJ. Clin. Invest.