Assembly and organization processes in DNA-directed colloidal crystallization

TitleAssembly and organization processes in DNA-directed colloidal crystallization
Publication TypeJournal Article
Year of Publication2009
AuthorsMacfarlane, RJ, Lee, B, Hill, HD, Senesi, AJ, Seifert, S, Mirkin, CA
JournalProceedings of the National Academy of Sciences of the United States of America
Pagination10493 - 10498
Date Published2009/06/30/
ISBN Number0027-8424

We present an analysis of the key steps involved in the DNA-directed assembly of nanoparticles into crystallites and polycrystalline aggregates. Additionally, the rate of crystal growth as a function of increased DNA linker length, solution temperature, and self-complementary versus non-self-complementary DNA linker strands (1- versus 2-component systems) has been studied. The data show that the crystals grow via a 3-step process: an initial "random binding'' phase resulting in disordered DNA-AuNP aggregates, followed by localized reorganization and subsequent growth of crystalline domain size, where the resulting crystals are well-ordered at all subsequent stages of growth.