Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis

TitleStochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis
Publication TypeJournal Article
Year of Publication2015
AuthorsCastellarnau, M, Szeto, GL, Su, H-W, Tokatlian, T, Love, JC, Irvine, DJ, Voldman, J
JournalSmall
Volume11
Issue4
Pagination489 - 498
Date Published2015/01/27/
Abstract

This study presents stochastic particle barcoding (SPB), a method for tracking cell identity across bioanalytical platforms. In this approach, single cells or small collections of cells are co-encapsulated within an enzymatically-degradable hydrogel block along with a random collection of fluorescent beads, whose number, color, and position encode the identity of the cell, enabling samples to be transferred in bulk between single-cell assay platforms without losing the identity of individual cells. The application of SPB is demonstrated for transferring cells from a subnanoliter protein secretion/phenotyping array platform into a microtiter plate, with re-identification accuracies in the plate assay of 96 +/- 2%. Encapsulated cells are recovered by digesting the hydrogel, allowing subsequent genotyping and phenotyping of cell lysates. Finally, a model scaling is developed to illustrate how different parameters affect the accuracy of SPB and to motivate scaling of the method to thousands of unique blocks.